. Block diagram of an HPLC–MS. A 3 component combination enters the HPLC. When part A elutes with the column, it enters the MS ion supply and ionizes to type the guardian ion and several fragment ions.Rotating the interior valve (proven in crimson) towards the inject position directs the mobile phase throughout the sample loop and on to the colum
. While in the load situation a sample loop—which is offered in a variety of sizes ranging from 0.five μL to five mL—is isolated through the mobile stage and open into the atmosphere. The sample loop is stuffed employing a syringe with a capability various instances that on the sample loop, with excessive sample exiting from the waste line.최
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